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Title 

Secretory expression of human αs₁-casein in Saccharomyces cerevisiae

Authors 

Yoo Kyeong KimDae Yeul YuKang Hyun AhSun YoonBong Hyun Chung

Publisher 

The Korean Society for Applied Microbiology

Issue Date 

1999

Citation 

Journal of Microbiology and Biotechnology, vol. 9, no. 2, pp. 196-200

Keywords 

Human α(s1)-caseinsecretionleader sequenceproteolysisSaccharomyces cerevisiae

Abstract 

A recombinant human α(s1)-casein was expressed as a secretory product in the yeast Saccharomyces cerevisiae. Three different leader sequences derived from the mating factor α1 (MFα1), inulinase, and human α(s1)- casein were used to direct the secretion of human α(s1)-casein into the extracellular medium. Among the three leader sequences tested, the native leader sequence of human α(s1)-casein was found to be the most efficient in the secretory expression of human α(s1)-casein, which implies that the native leader sequence of human α(s1)-casein might be used very efficiently for the secretory production of other heterologous proteins in yeast. The recombinant human α(s1)-casein was proteolytically cleaved as the culture proceeded. Therefore, an attempt was made to produce human α(s1)-casein using a S. cerevisiae mutant in which the YAP3 gene encoding yeast aspartic protease 3 (YAP3) was disrupted. After 72 h of culture, most of the human α(s1)-casein secreted by the wild type was cleaved, whereas more than 70% of the human α(s1)-casein secreted by yap3-disruptant remained intact. The results suggest that YAP3 might be involved in the internal cleavage of human α(s1)-casein expressed in yeast.

ISSN 

1017-7825

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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