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Title 

Vectors for rapid selection of intergrants with different plasmid copy numbers in the yeast Hansenula polymorpha DL1

Authors 

Michael O AgaphonovPolina M TrushkinaJung Hoon SohnEui Sung ChoiSang Ki RheeM D Ter-Avanesyan

Publisher 

Wiley-Blackwell

Issue Date 

1999

Citation 

Yeast, vol. 15, no. 0, pp. 541-551

Keywords 

Hansenula polymorphaplasmidtransformationmultiple integration

Abstract 

Plasmids with different selectable markers were constructed and used to transform the Hansenula polymorpha strain DLI. It was shown that, depending on the host mutant strain, the use of these plasmids enables rapid selection of transformants with plasmids integrated in law (1-2), moderate (6-9) or high (up to 100) copy numbers. The vectors and mutants described are potentially useful for the construction of efficient producers of heterologous proteins in N. polymorpha.

ISSN 

0749-503X

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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