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Title 

Screening and taxonomic characterization of D-amino acid aminotransferase-producing thermophiles

 

D-amino acid aminotransferase 활성보유 고온성미생물의 탐색 및 분류학적 특성 연구

Authors 

Mi Sun KwakSeung Goo LeeSang Cheol JeongSeung Hyun SuhJae Heung LeeYeong Joong JeonYoung Ho KimMoon Hee Sung

Publisher 

Korean Journal of Applied Microbiology and Biotechnology

Issue Date 

1999

Citation 

Korean Journal of Applied Microbiology & Biotechnology, vol. 27, no. 3, pp. 184-190

Keywords 

screeningD-amino acid aminotransferasetaxonomic characterizationthermophiles

Abstract 

To acquire an industrially useful biocatalyst for the enzymatic synthesis and production of various D-amino acids, 1,300 thermophiles isolated from the soil of Korea were analyzed for D-amino acid aminotransferase (DAAT) activity. The enzyme activity was found from 110 strains of isolated thermophiles revealing its wide occurrence in thermophiles. Enzyme activity and thermal stability of the D-AAT producers were compared. Finally we have selected four thermophiles as producers of potent biocatalysts for the D-amino acid production; two thermophiles, Bacillus sp. LK-1 and LK-2, having higher specific activity and two thermophiles, B. stearothermophilus KL-01 and Bacillus sp. KLS-01, having higher thermal stability than the D-AAT producers. Taxonomic and physiological characteristics of the four isolated thermophiles were described herein.

ISSN 

0257-2389

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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