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Title 

Incidence of hepatocellular carcinoma in transgenic mice expressing the hepatitis B virus X-protein

Authors 

Dae Yeul YuHyung Bae MoonJin Kyong SonSang Kyun JeongSeong Lan YuHee Sik YoonYong Mahn HanChul Sang LeeJung Sun ParkChul Ho LeeByung Hwa HyunSeishi MurakamiKyung Kwang Lee

Publisher 

Elsevier

Issue Date 

1999

Citation 

Journal of Hepatology, vol. 31, no. 0, pp. 123-132

Keywords 

HBV X-genehepatic tumorsproliferating cell nuclear antigentransgenic mice

Abstract 

Background/Aims: Chronic infection with hepatitis B virus is a high- risk factor for hepatocellular carcinoma in humans. The HBV X-protein, a multi-functional viral regulator, has been suspected to play a positive role in hepatocarcinogenesis, as demonstrated by the high incidence of hepatocellular carcinoma in HBx-expressing transgenic mice, although it is still controversial. The aim of this study was to generate transgenic mice expressing the HBV X-gene under authentic promoter control and to test whether the gene products can cause hepatic tumors. Methods: Three transgenic mouse lines were generated by microinjecting the X-gene construct into hybrid (C57BL/6xDBA) eggs. Gene expression was tested by protein and mRNA analyses. During an observation period of 18 months, mice were sacrificed and organs subjected to histologic examinations. Results: Grossly defined hepatocellular carcinomas reproducibly were observed in mice expressing the X-protein, which were investigated through six generations from the age of 11 to 18 months. Among 14 transgenic mice investigated from the age of 11 to 18 months, 12 were found to have hepatocellular carcinoma, grossly or microscopically. The lesion of the hepatocellular carcinoma disclosed a significant increase in the proliferating cell nuclear antigen in the nuclei. Conclusion: The incidence of hepatocellular carcinoma (86%) in our HBV X transgenic mice may be highly significant, since, except for one case, HBV X-gene transgenic mice produced in other laboratories did not develop liver tumor or any other pathologic phenomena.

ISSN 

0168-8278

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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