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Title 

Molecular cloning and analysis of sporulation-specific glucoamylase(SGA) gene of Saccharomyces diastaticus

Authors 

Dae Ook KangIn Kyu HwangWon Keun OhHyun Sun LeeSoon Cheol AhnBo Yeon KimTae Ick MheenJong Seog Ahn

Publisher 

Microbiological Society of Korea

Issue Date 

1999

Citation 

Journal of Microbiology, vol. 37, no. 1, pp. 35-40

Keywords 

S. DiastaticusSGA gene cloninggene disruptionsecretory protein

Abstract 

Sporulation-specific glucoamylase (SGA) gene was isolated from genomic library of Saccharomyces diastaticus 5114-9A by using glucoamylase non-producing mutant of S. diastaticus as a recipient. When the glucoamylase activities of culture supernatant, periplasmic, and intracellular fraction of cells transformed with hybrid plasmid containing SGA gene were measured, the highest activity was detected in culture supernatant. SGA produced by transformant and extracellular glucoamylase produced by S. diastaticus 5114-9A differed in enzyme characteristics such as optimum temperature, thermostability, and resistance to SDS and urea. But the characteristics of SGA produced by sporulating yeast cells and vegetatively growing transformants were identical.

ISSN 

1225-8873

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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