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Title 

Direct purification of lysozyme from hen egg white using high density mixed mode adsorbent

Authors 

Won Kyung KimBong Hyun Chung

Publisher 

The Korean Society for Applied Microbiology

Issue Date 

1999

Citation 

Journal of Microbiology and Biotechnology, vol. 9, no. 3, pp. 292-296

Keywords 

Lysozymehen egg white (HEW)mixed mode adsorbentexpanded bed

Abstract 

The high density mixed mode adsorbent known by the trade name of Mimo- AD was used to purify lysozyme directly from the hen egg white (HEW). The homogenized hen egg white was treated with the adsorbent in a stirred vessel for lysozyme adsorption, and then the adsorbent, easily separated from the HEW by sedimentation, was packed into a column. The remaining HEW and contaminant proteins were removed by washing with pH 11 distilled water in an expanded-bed state, and subsequently the elution was performed with pH 12 distilled water in a packed-bed state. By this simple and rapid adsorption, washing, and elution procedure, lysozyme was purified to >95% with an overall recovery yield of 66%. This process offers a great potential for industrial application by allowing the extraction of lysozyme while retaining the commercial value of HEW.

ISSN 

1017-7825

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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