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Title 

Suppression of proteolytic degradation of recombinant hirudin from Saccharomyces cerevisiae using the O₂-enriched air

Authors 

K Jagannadha RaoChul Ho KimBong Hyun ChungMyung Kuk KimSang Ki Rhee

Publisher 

Springer Verlag (Germany)

Issue Date 

1999

Citation 

Biotechnology Letters, vol. 21, no. 5, pp. 391-394

Keywords 

C-terminally truncated hirudinproteolytic degradationrecombinant hirudin

Abstract 

A novel process using O2-enriched air supply was used to suppress the C-terminal proteolytic degradation of recombinant hirudin (r-hirudin) from Saccharomyces cerevisiae. When dissolved O2 was controlled above 20% saturation level using normal air, inactive forms of C-terminally truncated hirudin were observed in culture broth from 48 h of fermentation. The use of O2-enriched air giving above 40% saturation of dissolved O2 suppressed the proteolytic degradation and hence the formation of truncated forms of inactive r-hirudin until 60 h of fermentation.

ISSN 

0141-5492

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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