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Title 

Purification and characterization of a bacteriocin produced by Lactococcus lactis subsp. lactis H-559 isolated from Kimchi

Authors 

Hun Joo LeeYun Jung JooChan Sun ParkSeung Ho KimIn Kyeong HwangJong Seog AhnTae Ick Mheen

Publisher 

Elsevier

Issue Date 

1999

Citation 

Journal of Bioscience and Bioengineering, vol. 88, no. 2, pp. 153-159

Keywords 

bacteriocinLactococcus lactis subsplactiskimchi

Abstract 

Lactic acid bacteria were isolated from kimchi and screened for bacteriocin production. Strain H-559, identified as Lactococcus lactis subsp, lactis, exhibited the strongest antibacterial activity among them and was active against pathogenic bacteria such as Listeria monocytogenes and Staphylococcus aureus as well as many lactic acid bacteria. The antimicrobial substance produced by L. lactis subsp. lactis H-559 was inactivated by α- chymotrypsin, and protease type IX and XIV and was confirmed to be a bacteriocin. The bacteriocin activity was stable from pH 2.0-11.0 and up to 10 min heating at 100°C. The bacteriocin was sequentially purified by ammonium sulfate precipitation, ion-exchange chromatography, and reversed- phase high-performance liquid chromatography (HPLC). Its molecular weight was determined to be 3343.7 Da by MALDI-mass spectrometry. Isoleucine was detected as the first N-terminal amino acid residue but the remaining amino acid sequence could not be determined by the Edman degradation method. It was different from other bacteriocins in terms of pH stability, molecular weight, amino acid composition, and the partial amino acid sequences of peptides obtained by acid hydrolysis.

ISSN 

1389-1723

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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