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Title 

Characterization of a biosurfactant, mannosylerythritol lipid produced from Candida sp. SY16

Authors 

Hee Sik KimByung Dae YoonDong Ho ChoungHee Moak OhT KatsuragiY Tani

Publisher 

Springer Verlag (Germany)

Issue Date 

1999

Citation 

Applied Microbiology and Biotechnology, vol. 52, no. 5, pp. 713-721

Keywords 

lipidsurfactantcandidafast atom bombardment mass spectrometryhigh performance liquid chromatographylipid analysisnonhumannuclear magnetic resonance spectroscopythin layer chromatographyCandida

Abstract 

One yeast strain, SY16, was selected as a potential producer of a biosurfactant, and identified as a Candida species. A biosurfactant produced from Candida sp. SY16 was purified and confirmed to be a glycolipid. This glycolipid-type biosurfactant lowered the surface tension of water to 29 dyne/cm at critical micelle concentration of 10 mg/l (1.5 x 10-5 M), and the minimum interfacial tension was 0.1 dyne/cm against kerosene. Thin-layer and high-pressure liquid chromatography studies demonstrated that the glycolipid contained mannosylerythritol as a hydrophilic moiety. The hydrophilic sugar moiety of the biosurfactant was determined to be β-D- mannopyranosyl-(1 → 4)-O-mesoerythritol by nuclear magnetic resonance (NMR) and fast atom bombardment mass-spectroscopy analyses. The hydrophobic moiety, fatty acids, of the biosurfactant was determined to be hexanoic, dodecanoic, tetradecanoic, and tetradecenoic acid by gas chromatography-mass spectroscopy. The structure of the native biosurfactant was determined to be 6-O-acetyl-2,3-di-O-alkanoyl-β-D-mannopyranosyl-(1 → 4)-O-meso-erythritol by NMR analyses. We newly determined that an acetyl group was linked to the C-6 position of the D-mannose unit in the hydrophilic sugar moiety.

ISSN 

0175-7598

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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