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Title 

Discovery of D-stereospecific dipeptidase from thermophilic Bacillus sp. BCS-1 and its application for synthesis of D-amino acid-containing peptide

Authors 

Dae Heoun BaekSeok Joon KwonJin Seo ParkSeung Goo LeeTae Ick MheenMoon Hee Sung

Publisher 

The Korean Society for Applied Microbiology

Issue Date 

1999

Citation 

Journal of Microbiology and Biotechnology, vol. 9, no. 5, pp. 646-649

Keywords 

Screeningthermophilic BacillusD-stereospecific dipeptidaseenzymatic synthesisD-amino acid-containing peptide

Abstract 

A thermophilic bacterium producing D-stereospecific dipeptidase was isolated from Korean soil samples. The enzyme hydrolyzed the peptide bond between D-alanyl-D-alanine (D-Ala-D-Ala). The isolated bacterial strain was rod shaped, gram-positive, motile, and formed an endospore. Morphological and physiological characteristics suggested this microorganism a thermophilic Bacillus species, and was named as Bacillus sp. BCS-1. The production of D- stereospecific dipeptidase was growth-associated and optimal at 55°C. The enzyme was applied for the synthesis of D-amino acid-containing peptide, N- benzyloxycarbonyl-L-aspartyl-D-alanine benzyl ester (Z-L-Asp-D-AlaOBzl), as a model reaction. A thermodynamically controlled synthesis of Z-L-Asp-D-AlaOBzl was achieved in an organic solvent.

ISSN 

1017-7825

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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