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Title 

Heterozygous ras mutations are preserved in serially passaged human tumor xenografts and established cell lines

Authors 

Keiko MasudaHiroshi KijimaHwan Mook KimSang Bae HanYasuyuki OhnishiNobuko SawaYoshiro OshikaTetsuji TokunagaTakashi TsuchidaYoshiyuki AbeToshiyasu HamanaHiroyuki MatsubayashiHitoshi YamazakiNorikazu TamaokiYoshito UeyamaMasato Nakamura

Publisher 

Spandidos Publications

Issue Date 

1999

Citation 

Oncology Reports, vol. 6, no. 0, pp. 741-745

Keywords 

ras oncogenepoint mutationxenograftcell lineserial passage

Abstract 

We examined c-K-ras gene point mutations in human tumor xenografts and established cell lines as markers of genetic stability. Our previous study demonstrated the stability of c-K-ras gene mutations in human primary neoplasms and their tumor xenografts through serial passages in mice. In this study, we established 27 human cell lines derived from various human tumor xenografts in nude mice. Point mutation of the c-K-ras gene at codon 12 was found in 29.6% (8/27) of the cell lines, as well as in 29.6% (8/27) of the xenografts. The eight ras-mutated cell lines were derived from corresponding tumor xenografts carrying the ras mutation. Heterozygous ras gene mutation was confirmed in seven of the eight ras-mutated cell lines, as well as their corresponding xenografts. The incidence, type and heterozygosity of the c-K-ras gene mutation showed no discrepancies between the original xenografts and the established cell lines. From these findings, we concluded that point mutation of the c-K-ras gene was very stable in human tumor xenografts and established cell lines derived from the xenografts.

ISSN 

1021-335X

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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