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Title 

Isolation of xylanase-producing thermo-tolerant Bacillus sp. and its enzyme production

 

Xylanase를 생산하는 내열성 Bacillus 속 균주의 분리와 효소생산 조건

Authors 

Young Seo ParkMi Young KangHak Gil ChangGwi Gun ParkJong Back KangJung Kee LeeTae Kwang Oh

Publisher 

Korean Journal of Applied Microbiology and Biotechnology

Issue Date 

1999

Citation 

Korean Journal of Applied Microbiology & Biotechnology, vol. 27, no. 5, pp. 370-377

Keywords 

xylanaseBacillus pumilusenzyme production

Abstract 

Thermo-tolerant bacterium producing the xylanase was isolated from soil and identified as Bacillus pumilus. This strain, named Bacillus pumilus TX703, was able to grow and produce xylanase at the culture temperature of 50°C. The maximum xylanase production was obtained when 1% (w/v) birchwood xylan and 1% (w/v) soytone were used as carbon source and nitrogen source, respectively. The biosynthesis of xylanase was under the catabolite repression induced by glucose in the culture medium, and it was completely inhibited in the presence of 0.2% (w/v) glucose. The maximum activity of xylanase was observed from pH 8.0 to 9.0 and from 50 to 60°C and the enzyme was highly heat-stable, whose activity remained was over 50% at 80°C, and was quite stable from pH 5.0 to 10.0.

ISSN 

0257-2389

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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