상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

Efficient expression and characterization of hepatitis B virus preS2 antigen in Escherichia coli

Authors 

Young Jun KangYoung Shin KimHyang Suk HurHee Sun KimHyo Jeong Hong

Publisher 

Springer Verlag (Germany)

Issue Date 

1999

Citation 

Biotechnology Letters, vol. 21, no. 0, pp. 375-380

Keywords 

Escherichia coliExpressionhepatitis B viruspreS2 antigen

Abstract 

The complete (encoding 55 amino acids, aa) or partial (encoding aa 1-26) preS2 region gene of hepatitis B virus (HBV) was fused to the 3'-end of glutathion-S-transferase (GST) gene and expressed under the control of the inducible tac promoter in Escherichia coli at 37°C. The fusion protein with the complete preS2 region was moderately expressed (8%) while the protein with the N-terminal 26 aa was expressed at a higher level, yielding about 20% of the total cellular proteins. The GST-preS2 (aa 1-26) protein, which contains the immunodominant epitope, was produced form the soluble protein fraction of the recombinant bacteria and purified by affinity chromatography using glutathione-agarose column. The purified preS2 fusion protein showed the antigenicity of preS2, as assessed by indirect and competitive ELISAs.

ISSN 

0141-5492

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


There are no files associated with this item.
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)