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Title 

Generation of human fab monoclonal antibodies against preS1 of hepatitis B virus using repertoire cloning

Authors 

In Hak ChoiSae Gwang ParkJun Ho ChungIk Jung KimHyo Jeong Hong

Publisher 

Mary Ann Liebert

Issue Date 

1998

Citation 

Hybridoma, vol. 17, no. 6, pp. 535-540

Keywords 

hepatitis b antigenimmunoglobulin f(ab) fragmentmonoclonal antibodyprotein pres1virus proteinantibody productionantibody titerenzyme linked immunosorbent assayhepatitis bhuman

Abstract 

Human monoclonal antibodies (MAbs) have considerable potential in the prevention and treatment of many viral diseases. A combinatorial antibody library of heavy (Fd)- and light-chain genes derived from peripheral blood lymphocytes of a volunteer with high antibody titer to preS1 of HBV was constructed and expressed on the surface of filamentous phages. The library contained 7 x 109 independent clones. A phage antibody population from the third panning against preS1 was converted to one expressing soluble Fabs by removal of the g3 sequences from the pComb3 phagemid vector and subsequent transformation into E. coli TG1 cells. Screening of the library led to the identification of two clones, 3DW and 8GW, showing high reactivity toward preS1. The authenticity of the Fabs was confirmed by immunoblot analysis which yielded ~60 and ~30 kDa bands under nonreducing and reducing conditions, respectively. The soluble Fabs of 3DW and 8GW exhibited relative affinities of 6 x 105 and 8 x 106 M-1, respectively. The sequencing results demonstrate that all Fd sequences belong to subgroup II and all light chain sequences belong to subgroup I. There are differences in CDR length and composition, especially in the FW3 and CDR3 regions of the heavy- and light- chain genes. These human Fab MAbs specific to preS1, generated from a combinatorial library, represent prototypes of passive immunotherapy candidates for viral hepatitis B.

ISSN 

0272-457X

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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