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Title 

High frequency plant regeneration from immature ovule-derived embryogenic cell suspension cultures of Chelidonium majus var. asiaticum

Authors 

Suk Weon KimByung Whan MinJang Ryol Liu

Publisher 

Springer Verlag (Germany)

Issue Date 

1999

Citation 

Plant Cell Tissue and Organ Culture, vol. 56, no. 0, pp. 125-129

Keywords 

PapaveraceaeSomatic embryoSomatic embryogenesis

Abstract 

Culture conditions for high frequency plant regeneration via somatic embryogenesis in cell suspension cultures of Chelidonium majus var. asiaticum are described. Immature ovules formed embryogenic calluses at a frequency of 40% when cultured on Murashige and Skoog (MS) medium supplemented with 4,52 μM 2,4-dichlorophenoxyacetic acid (2,4-D). The optimum ovule size for embryogenic callus formation ranged from 1 to 1.5 mm in length. Cell suspension cultures were established from embryogenic calluses using MS liquid medium containing 4.52 μM 2,4-D. Upon plating onto MS basal medium, cell aggregates from cell suspension cultures produced somatic embryos which then developed into plantlets. Regenerated plantlets were transplanted to potting soil and grown to maturity in a growth chamber.

ISSN 

0167-6857

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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