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Title 

Effect of specific production rate of recombinant protein on multimerization of plasmid vector and gene expression level

Authors 

Vibhor SaraswatDae Young KimJee Won LeeYoung Hoon Park

Publisher 

Wiley-Blackwell

Issue Date 

1999

Citation 

FEMS Microbiology Letters, vol. 179, no. 0, pp. 367-373

Keywords 

Recombinant human interleukin-2Specific production ratePlasmid multimerizationEscherichia coli

Abstract 

In fed-batch cultures of recombinant Escherichia coli BL21(DE3)[pT7-G3IL2] at high cell concentration, the post-induction specific growth rate was carefully regulated by controlled medium feed to maximize the synthesis level of recombinant fusion interleukin-2, G3.IL-2. A maximum concentration of G3.IL-2 (11.25 g l-1) was achieved in the induced recombinant culture growing at the rate of 0.056 h-1. A steep decrease in the expression level of G3.IL-2 was observed at the post-induction specific growth rates higher than its optimal value (0.056 h-1). In the induced recombinant cultures, plasmid multimerization was observed and highly dependent on specific growth and production rate: a higher post-induction specific growth rate and an increased specific production rate tended to significantly promote it much further. Moreover, plasmid stability was found to decrease rapidly in a faster growing culture.

ISSN 

0378-1097

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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