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Title 

Transgenic tobacco plants expressing the bacterial levansucrase gene show enhanced tolerance to osmotic stress

Authors 

Jeong Mee ParkSuk Yoon KwonKi Bang SongJu Won KwakSuk Bae LeeYoung Woo NamJeong Sheop ShinYoung In ParkSang Ki RheeKyung Hee Paek

Publisher 

The Korean Society for Applied Microbiology

Issue Date 

1999

Citation 

Journal of Microbiology and Biotechnology, vol. 9, no. 2, pp. 213-218

Keywords 

Levansucraseosmotic stresstobaccotransgenic plantZymomonas mobilis

Abstract 

Fructans are polyfructose molecules that function as nonstructural storage carbohydrates in several plants. In addition, it has been suggested that, due to their solubility, they can play an important role in helping plants survive periods of osmotic stress. In order to study the effect of levan synthesis on plant growth, the coding region of the levansucrase gene, which was isolated from Zymomonas mobilis, was introduced into tobacco plants using Agrobacterium tumefaciens-mediated transformation. The presence of the levansucrase gene in transgenic plants was verified by genomic DNA gel blot analysis. RNA gel blot and immunoblot analyses showed an accumulation of the corresponding transcript and protein product of the bacterial levansucrase gene in transgenic plants. Furthermore, a thin layer chromatography analysis revealed that fructans were synthesized and deposited in transgenic tobacco plants. When T1 seeds were germinated and grown under polyethylene glycol- mediated drought stress or cold stress, the transgenic seedlings displayed a substantially higher level of growth than that of untransformed plants. These results suggest that fructans may play a significant role in the tolerance of plants under osmotic stress.

ISSN 

1017-7825

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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