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Title 

p53 Negatively regulates cdc2 transcription via the CCAAT-binding NF-Y transcription factor

Authors 

Jean Ho YunHee Don ChaeHyon E ChoyJong Kyeong ChungHyang Sook YooMoon Hi HanDeug Young Shin

Publisher 

American Society for Biochemistry and Molecular Biology

Issue Date 

1999

Citation 

Journal of Biological Chemistry, vol. 274, no. 42, pp. 29677-29682

Keywords 

cyclineprotein p53transcription factorcell cyclecontrolled studygenetic transcriptionpolymerase chain reactionprotein bindingprotein DNA bindingsequence analysis

Abstract 

The p53 tumor suppressor protein regulates the transcription of regulatory genes involved in cell cycle arrest and apoptosis. We have reported previously that inducible expression of the p53 gene leads to the cell cycle arrest both at G1 and G2/M in association with induction of p21 and reduction of mitotic cyclins (cyclin A and B) and cdc2 mRNA. In this study, we investigated the mechanism by which p53 regulates transcription of the cdc2 gene. Transient transfection analysis showed that wild type p53 represses whereas various dominant negative mutants of p53 increase cdc2 transcription. The cdc2 promoter activity is not repressed in cells transfected with a transactivation mutant, p53(22/23). An adenovirus oncoprotein, E1B-55K inhibits the p53-mediated repression of the cdc2 promoter, while E1B-19K does not. Since the cdc2 promoter does not contain a TATA sequence, we performed deletion and point mutation analyses and identified the inverted CCAAT sequence located at -76 as a cis-acting element for the p53-mediated regulation. We found that a specific DNA-protein complex is formed at the CCAAT sequence and that this complex contains the NF-Y transcription factor. Consistently, a dominant negative mutant of the NF-YA subunit, NF-YAm29, decreases the cdc2 promoter, and p53 does not further decrease the promoter activity in the presence of NF-YAm29. These results suggest that p53 negatively regulates cdc2 transcription and that the NF-Y transcription factor is required for the p53-mediated regulation.

ISSN 

0021-9258

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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