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Title 

Molecular cloning and functional expression of a phospholipase D from cabbage (Brassica oleracea var. capitata)

Authors 

Dong Uk KimTae Young RohJi Eun LeeJoo Yoon NohYoung Joo JangKwang Lae HoeHyang Sook YooMyung Un Choi

Publisher 

Elsevier

Issue Date 

1999

Citation 

Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, vol. 1437, no. 3, pp. 409-414

Keywords 

Phospholipase DcDNA sequenceExpression(Brassica oleracea var. capitata)

Abstract 

We cloned and expressed a full-length cDNA encoding a phospholipase D of type α (PLDα) from cabbage. Analysis of the cDNA predicted an 812-amino- acid protein of 92.0 kDa. The deduced amino acid sequence of cabbage PLD has 83% and 80% identity with Arabidopsis PLDα and castor bean PLD, respectively. Expression of this cDNA clone in E. coli shows a functional PLD activity similar to that of the natural PLD.

ISSN 

1388-1981

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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