상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

DNA-induced conformational change of Gaf1, a novel GATA factor in Schizosaccharomyces pombe

Authors 

Mi Sun WonKwang Lae HoeYong Sik ChoKyung Bin SongHyang Sook Yoo

Publisher 

National Research Council Canada

Issue Date 

1999

Citation 

Biochemistry and Cell Biology, vol. 77, no. 2, pp. 127-132

Keywords 

fateur GATASchizosaccharomyces pombeConformational changeDNA-bindingGATA factortranscription factortranscription factor gaf1unclassified drugalpha helixconformational transitionfungal genetics

Abstract 

A novel GATA factor in Schizosaccharomyces pombe, Gaf1, containing one zinc-finger motif was studied for conformational change that was induced by DNA-binding. Gaf1 was shown to bind to the upstream activation sequence of a gene in Saccharomyces cerevisiae containing GATA element by gel mobility shift assay. Circular dichroism spectra of Gaf1 indicated an increase of α- helix content of Gaf1 occurred upon binding to the upstream activation sequence. These results suggest that the binding of Gaf1 to the GATA element is required for the conformational change that may precede transactivation of the target gene(s).

ISSN 

0829-8211

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


There are no files associated with this item.
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)