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Title 

Induction of cytotoxic T lymphocytes with peptides in vitro: identification of candidate T-cell epitopes in hepatitis B virus X antigen

Authors 

Mi Kyung ChungHee Sik YoonSung Shik MinHee Gu LeeYoung Jae KimTae Gyu LeeJong Soon LimChang Min KimSue Nie Park

Publisher 

Raven Press Publishers

Issue Date 

1999

Citation 

Journal of Immunotherapy, vol. 22, no. 4, pp. 279-287

Keywords 

cd8 antigenepitopehepatitis b antigenHLA A2 antigensynthetic peptideantigen presentationbinding affinitycontrolled studycytotoxic t lymphocytehepatitis b

Abstract 

Cytotoxic T lymphocytes (CTL) have been suggested to contribute to viral clearance during hepatitis B virus (HBV) infection. To induce effective CTL against vital infection by peptide vaccination, it is essential to identify the epitope peptides recognized by CTL. Here, 15 peptide sequences that contain HLA-A2.1-restricted CTL binding consensus motif were identified on hepatitis B virus X (HBx) protein and synthesized for further characterization. In the binding assay, 8 of 15 synthetic peptides enhanced the expression of HLA-A2.1 molecules on the surface of T2 cells, a human transport-associated antigen processing-deficient cell line. This result implies that these eight peptides are able to bind to the HLA-A2.1 molecules. These peptides were further tested for their ability to activate CTL from peripheral blood mononuclear cells (PBMCs) isolated from HBV chronic carriers. Five of eight tested peptides activated PBMC-derived T cells, resulting in the lysis of the target T2 cells pulsed with the same peptide. Furthermore, the CTL responses to HBx antigen in HBV chronic carriers were shown to be polyclonal, multispecific, and mediated mainly by CD8+ T cells. In contrast, these responses were not detected in uninfected healthy blood donors. Although the five CTL epitope peptides identified in this study have not been proven to be the naturally processed epitopes in HBV-infected hepatocytes, they could be candidates for peptide-based immunotherapy against HBV infection.

ISSN 

1053-8550

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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