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Title 

In vitro activation of yeast pro-carboxypeptidase Y expressed as inclusion bodies in Escherichia coli

Authors 

Moon Sun HahmJung Hoon BaeEui Sung ChoiBong Hyun Chung

Publisher 

Springer Verlag (Germany)

Issue Date 

1999

Citation 

Biotechnology Letters, vol. 21, no. 0, pp. 881-885

Keywords 

E. coliH. polymorphain vitro activationpro-carboxypeptidase Yproteinase K

Abstract 

The genes encoding pro-carboxypeptidase Ys (pro-CPYs) from Saccharomyces cerevisiae and Hansenula polymorpha were cloned and expressed in Escherichia coli. Most of the expressed pro-CPY was present in the form of inclusion bodies, accounting for about 35% of the total cellular protein. The inclusion bodies solubilized in 6 M guanidinum chloride were renatured by dilution 1:60 into renaturation buffer. Further refolding and activation were followed by the addition of 60 μg ml-1 proteinase K into the diluted solution. The specific activities of in vitro activated S. cerevisiae and H. polymorpha CPYs were found to be similar, representing about 25% of that of native S. cerevisiae CPY.

ISSN 

0141-5492

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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