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Title 

Microcystin production by Microcystis aeruginosa in a phosphorus-limited chemostat

Authors 

Hee Mock OhSeog June LeeMin Ho JangByung Dae Yoon

Publisher 

American Society for Microbiology

Issue Date 

2000

Citation 

Applied and Environmental Microbiology, vol. 66, no. 1, pp. 176-179

Keywords 

cyanoginosinphosphorusbacterial growthbacterium culturechemostatmicrocystis aeruginosanonhumantoxin analysisculture mediamicrocystins

Abstract 

The production of microcystins (MC) from Microcystis aeruginosa UTEX 2388 was investigated in a P-limited continuous culture. MC (MC-LR, MC-RR, and MC-YR) from lyophilized M. aeruginosa were extracted with 5% acetic acid, purified by a Sep- Pak C18 cartridge, and then analyzed by high-performance liquid chromatography with a UV detector and Nucleosil C18 reverse- phase column. The specific growth rate (μ) of M. aeruginosa was within the range of 0.1 to 0.8/day and was a function of the cellular P content under a P limitation. The N/P atomic ratio of steady-state cells in a P-limited medium varied from 24 to 15 with an increasing μ. The MC-LR and MC-RR contents on a dry weight basis were highest at μ of 0.1/day at 339 and 774 μg g-1, respectively, while MC-YR was not detected. The MC content of M. aeruginosa was higher at a lower μ, whereas the MC-producing rate was linearly proportional to μ. The C fixation rate at an ambient irradiance (160 microeinsteins m-2 s-1) increased with μ. The ratios of the MC-producing rate to the C fixation rate were higher at a lower μ. Accordingly, the growth of M. aeruginosa was reduced under a P limitation due to a low C fixation rate, whereas the MC content was higher. Consequently, increases in the MC content per dry weight along with the production of the more toxic form, MC-LR, were observed under more P-limited conditions.

ISSN 

0099-2240

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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