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Title 

Extracellular secretion of levansucrase from Zymomonas mobilis in Escherichia coli

Authors 

K H JangJeong Woo SeoKi Bang SongChul Ho KimSang Ki Rhee

Publisher 

Springer

Issue Date 

1999

Citation 

Bioprocess Engineering, vol. 21, no. 5, pp. 453-458

Keywords 

levansucraseescherichia coliextracellular spacezymomonas mobilisEscherichia coli

Abstract 

Secretion of levansucrase from Zymomonas mobilis in Escherichia coli by glycine supplement was investigated. A significant amount of levansucrase (about 25% of total activity) was found in intact whole-cells. Cell fractionation experiments showed that levansucrase was found both in the periplasmic space and in the cytoplasmic fraction of E. coli. None or only trace amounts of levansucrase was detected in the extracellular culture broth at 24 h of cultivation and it accrued with the increasing concentration of glycine in the culture medium and duration of the culture period. Optimal glycine concentration for the maximum secretion of levansucrase was in the range of 0.8-1%, in which approximately 20-50% of levansucrase was released into the extracellular fraction at 24 h of cultivation, although glycine retarded the bacterial growth.

ISSN 

0178-515X

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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