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Title 

Expression of Schwanniomyces occidentalis α-Amylase gene in Saccharomyces cerevisiae var. diastaticus

Authors 

Jeong Nam ParkDong Jun ShinHee Ok KimDong Ho KimHwang Hee Blaise LeeSoon Bae ChunSuk Bai

Publisher 

The Korean Society for Applied Microbiology

Issue Date 

1999

Citation 

Journal of Microbiology and Biotechnology, vol. 9, no. 5, pp. 668-671

Keywords 

Schwanniomyces occidentalis α-amylase geneyeast integrating vectormitotic stabilitySaccharomyces cerevisiae var. diastaticus

Abstract 

The gene encoding Schwanniomyces occidentalis α-amylase (AMY) was introduced into Saccharomyces cerevisiae var diastaticus which secreted only glucoamylase, by using a linearized yeast integrating vector to develop stable strains with a capability of secreting α-amylase and glucoamylase simultaneously. A dominant selectable marker, the geneticin (G418) resistance gene (Gt), was cloned into a vector to screen wild-type diploid transformants harboring the AMY gene. The amylolytic activities of transformants were about 3-7 times higher than those of the recipient strains. When grown in nonselective media, the transformants with the linearized integrating vector containing the AMY gene exhibited almost all of the mitotic stability after 100 generations.

ISSN 

1017-7825

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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