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Title 

Isolation of a high-yield mutant strain for L-proline production and its fermentation conditions

Authors 

Wuk Sang RyuHyung Wook JangKyoung Hee ChoSoon Jae ChangYeon Woo RyuYoung Hoon Park

Publisher 

The Korean Society for Applied Microbiology

Issue Date 

1999

Citation 

Journal of Microbiology and Biotechnology, vol. 9, no. 5, pp. 613-618

Keywords 

L-prolineL-histidine auxotrophsulfaguanidine resistant mutantproline analog resistant mutantγ-glutamyl kinase

Abstract 

L-Proline-producing mutant strains were developed by exposing L-glutamic acid-producing bacteria to N-metyl-N-nitro-nitrosoguanidine and UV irradiation. A L-histidine auxotroph of Corynebacterium acetoacidophilum RYU3161 (KCTC 0616BP), which was resistant to sulfaguanidine and proline analogs (DHP, AZC, TAC), was isolated. The activity of the mutant strain's γ-glutamyl kinase was 45% higher than that of the parent strain. The optimum level of L-histidine for production of L-proline was 0.16 g/l. In a 5-1 jar fermenter, the mutant strain produced L-proline at a high concentration (35 g/l) level within 48 h of cultivation.

ISSN 

1017-7825

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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