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Title 

High-level expression of a recombinant thermostable phytase in Bacillus subtilis

Authors 

Young Ok KimJung Kee LeeByung Chul OhTae Kwang Oh

Publisher 

Japan Society for Bioscience, Biotechnology and Agrochemistry

Issue Date 

1999

Citation 

Bioscience Biotechnology and Biochemistry, vol. 63, no. 12, pp. 2205-2207

Keywords 

Bacillus subtilisphytasephytateoverexpression

Abstract 

An efficient expression system was developed in Bacillus subtilis for the large scale production of phytase. The phytase gene with a native promoter derived from Bacillus amyloliquefaciens was cloned in the Bacillus expression vector pJH27 under a strong BJ27 promoter and its expression was optimized. The expression of the phytase gene occurred during late exponential growth and the extracellular phytase production was 2.0 units/ml, which constituted over 90% of the total protein. The yield was 100-fold higher than wild type Bacillus amyloliquefaciens DS11.

ISSN 

0916-8451

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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