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Title 

Two carbohydrate recognition domains of Hyphantria cunea lectin bind to bacterial lipopolysaccharides through O-specific chain

Authors 

Sang Woon ShinDoo Sang ParkSun Chang KimHo Yong Park

Publisher 

Elsevier

Issue Date 

2000

Citation 

FEBS Letters, vol. 467, no. 1, pp. 70-74

Keywords 

Carbohydrate recognition domainHyphantria cunea lectinInsect immunityLipopolysaccharide bindingbacterium lipopolysaccharidelectinO antigenamino acid sequencebacterial infectionbinding site

Abstract 

We previously identified a novel lectin cDNA from the fall webworm [Shin et al. (1998) Insect Biochem. Mol. Biol. 28, 827-837], which encodes two carbohydrate recognition domains (CRD-N and CRD-C) and is up-regulated following bacterial challenge. The lipopolysaccharide (LPS) binding activities of the recombinant CRD-N and CRD-C (rCRD-N and rCRD-C) were investigated by enzyme-linked immunosorbent assay. The LPS binding of rCRD-N and rCRD-C was pH-dependent: at pH below 6.0, they show a higher binding ability to LPS. The binding of the rCRD-N was inhibited by both D-mannose and N-acetyl-D-glucosamine, whereas the binding of the rCRD-C was inhibited only by D-mannose. The binding of both rCRD-N and rCRD-C to Escherichia coli was mainly mediated through the O-specific chain.

ISSN 

0014-5793

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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