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Title 

Cloning and sequencing of the rph gene encoding RNase PH from Legionella pneumophila

Authors 

Se Jin KimJong-Seok LimNicholas P CianciottoYong Kyung Choe

Publisher 

Microbiological Society of Korea

Issue Date 

1999

Citation 

Journal of Microbiology, vol. 37, no. 4, pp. 218-223

Keywords 

Exo-ribonucleaseLegionella pneumophilaRNase phrph gene

Abstract 

Legionella pneumophila, the cause of Legionnaires' disease, is able to survive intracellularly in eukaryotic cells such as monocytes, macrophages, and protozoan organisms. During protein biosynthesis, the rph gene encodes ribonuclease (RNase) PH which functions as a phosphorolytic nuclease that removes nucleotides following the CCA terminus of tRNA and as a nucleotidyl-transferase which adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates. In this study, the rph gene was screened in pUC19 library employing a DNA probe which was constructed from PCR based on a consensus pattern of multiple alignment of RNase PH. The encoded protein consists of 235 amino acid residues with a calculated molecular weight of 26,112 Daltons. The RNase PH signature domains are completely conserved.

ISSN 

1225-8873

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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