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Title 

High frequency shoot induction and plant regeneration from cotyledonary hypocotyl explants of Cucumber(Cucumis sativus L.) seedlings

Authors 

Jae Whune KimSu Kyung HanSuk Yoon KwonHeang Soon LeeYong Pyo LimJang Ryol LiuSang Soo Kwak

Publisher 

Elsevier

Issue Date 

2000

Citation 

Journal of Plant Physiology, vol. 157, no. 1, pp. 136-139

Keywords 

cucumis sativushypocotyl segmentplant regenerationshoot induction

Abstract 

A method of high frequency shoot induction was established using excised hypocotyl segments bearing cotyledons (referred to as cotyledonary hypocotyl explants) of cucumber (Cucumis sativus L.) seedlings. Adventitious shoots were obtained from the upper excised hypocotyls of cucumber seedlings incubated on Murashige and Skoog (MS) medium containing 2.0 mg/L zeatin. The effects of cotyledon size and hypocotyl length of seedlings on shoot organogenesis were also examined. A higher frequency (80-90 %) of adventitious shoots was obtained from explants of 3- and 5-day-old seedlings with either 1 cotyledon or 2 half-cotyledons, and a short hypocotyl length (2 mm). However, explants with long hypocotyls and 2 cotyledons produced fewer shoots. Adventitious shoots from cotyledonary hypocotyl explants were removed and rooted in MS medium containing 1.0 mg/L IAA. The rooted plantlets developed into normal plants after acclimatization to ambient humidity levels.

ISSN 

0176-1617

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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