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Title 

Development of RFLP markers from silkworm(bombyx mori)

 

누에의 RFLP marker 개발

Authors 

Jin Sung LeeJae Sam HwangSang Mong LeeSeung Hyun SungYong Sung KimDong Sang Suh

Publisher 

The Genetics Society of Korea

Issue Date 

1999

Citation 

Korean Journal of Genetics, vol. 21, no. 4, pp. 319-327

Keywords 

linkage mapplasmid genomic libraryRFLPsilkworm

Abstract 

To construct RFLP linkage map of silkworm (Bombyx mori), as a preliminary step, we assessed the efficiency for single or low copy sequences from silkworm genomic DNA. A genomic DNA plasmid library was constructed using EcoRI-digested 0.3 to 5 kb DNA fragments of II-111 strain. As results of two step screening (reverse colony hybridization and reverse southern hybridization) using a total genomic DNA as a probe, about 6% (70/1,200) of clones examined was estimated single or low copy sequences. At least 80% of total clones contained middle or high repetitive sequences. These results indicate that B. mori genome has a lot of high repetitive sequences. However, analysis for its precise copy number in the genome remains to be elucidated. The obtained 70 single-sequences will be useful molecular markers to study silkworm genomic analysis in the near future, although it has low efficiency of single copy sequence for constructing linkage map.

ISSN 

0254-5934

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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