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Title 

Characterization of KI-24, a novel murine monoclonal antibody with specific reactivity for the human immunodeficiency virus-1 p24 protein

Authors 

Song Yub ShinJung Hyun ParkMyung Kyu LeeSo Youn JangKyung Soo Hahm

Publisher 

Springer Verlag (Germany)

Issue Date 

2000

Citation 

Journal of Biochemistry and Molecular Biology, vol. 33, no. 1, pp. 92-95

Keywords 

B-cell epitopeHIV-1 p24(202-221)HIV-1 p24 proteinmonoclonal antibody

Abstract 

The HIV-1 p24(202-221) sequence ETINNEEEWDRVHPV HAGP contains a B-cell epitope with the earliest immune response and the highest antibody titer against anti-mouse sera obtained by immunization with p24 antigens. A novel mouse monoclonal antibody (mAb) was generated against the immunodominant B-cell epitope of the HIV-1 p24 capsid protein, p24(202-221). BALB/c mice were immunized with the four branched multiple antigenic peptide (MAP) containing the HIV-1 p24(202-221) sequence, and antibody-secreting hybridoma were produced by fusion of mouse splenocytes with P3X63Ag8.653, mouse myeloma cells. One clone which produced the antigen-specific mAb named KI-24 (Isotype IgG1, light chain: κ) was identified. mAb KI-24 was highly specific for both the p24(202-221) and p24 proteins when analyzed by ELISA and Western blotting. Since p24(202-221) also contains a cytotoxic T-lymphocyte epitope, this specfic peptide epitope and the monoclonal antibody with specific reactivity against the p24 protein and p24(202-221) can be used in peptide vaccine development and p24 antigen detection from HIV patients.

ISSN 

1225-8687

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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