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Title 

Improvement of intact human lipocortin-I production in Saccharomyces cerevisiae by inhibiting proteolysis

Authors 

Won A ChoiGui Hwan OhKwang Hyun AhBong Hyun Chung

Publisher 

Elsevier

Issue Date 

2000

Citation 

Journal of Bioscience and Bioengineering, vol. 89, no. 1, pp. 77-80

Keywords 

human lipocortin-IproteolysisL-lysineL- arginineendoproteasesSaccharomyces cerevisiae

Abstract 

Human lipocortin-I (hLC1), when was expressed as a secretory product in Saccharomyces cerevisiae, was cleaved to a significant extent by endoproteolytic processing, resulting in the accumulation of des1-26-hLC1 in the culture supernatant. This proteolytic cleavage was inhibited significantly by the addition of high concentrations of L-arginine and L- lysine, with a resultant marked improvement in the yield of intact hLC1. When the hLC1 was expressed in S. cerevisiae mutants deficient in one or two of the following endoproteases, Kex2p, Mke7p and Yps1p (Yap3p), the mutants exhibited no reduction in the extent of hLC1 proteolysis, indicating that these endoproteases are not involved in the proteolytic cleavage of hLC1.

ISSN 

1389-1723

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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