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Title 

Development of new adherent mutant from human myelomaderived cell line: in vitro model of anaplastic transformation of myeloma

Authors 

Kyung Cheon JungChan Sik ParkHae Won SohnMyeong Cherl KookDo Hyun ChungYang Kyu ChoiByung Hwa HyunIm Soon LeeYoung Mee BaeHyung Geun SongSeong Hoe Park

Publisher 

Springer Verlag (Germany)

Issue Date 

1999

Citation 

Molecules and Cells, vol. 9, no. 6, pp. 657-661

Keywords 

anaplastic myelomaIM-9/ADin vitro model

Abstract 

Anaplastic myeloma is a rare but distinct, biologically aggressive variant of myeloma which usually results from dedifferentiation or anaplastic transformation of the myeloma cells. The molecular mechanisms that determine the biologic behavior of anaplastic myeloma and effective treatment modalities have not been well known due to lack of in vitro models. In the present study, we have developed an anaplastically transformed mutant from a human myeloma-derived cell line. In the process of long-term culture of the myeloma-derived IM-9 cell line in low serum and nutrient conditions, an adherent mutant line was developed and named IM-9/ AD. This mutant cell line displayed several characteristics resembling anaplastic myeloma such as: 1, large cells with large vesicular nucleus and prominent nucleolus, multinuclearity and high mitotic figures; 2, loss of leukocyte-associated antigens; and 3, higher tumorigenecity in scid mice than its parental cell line. This newly developed mutant cell line may serve as a readily available in vitro model to investigate the biology of anaplastic myeloma.

ISSN 

1016-8478

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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