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Title 

Screening of microorganisms producing esterase for the production of (R)-β-acetylmercaptoisobutyric acid from methyl (R,S)-β-acetylmercaptoisobutyrate

Authors 

Boyapati GokulJe Hyuk LeeKi Bang SongT PandaSang Ki RheeChul Ho Kim

Publisher 

Springer Verlag (Germany)

Issue Date 

2000

Citation 

Biotechnology and Bioprocess Engineering, vol. 5, no. 1, pp. 57-60

Keywords 

(R)-β-acetylmercaptoisobutyric acidPseudomonas sp.esteraseenzymatic resolution

Abstract 

(R)-β-acetylmercaptoisobutyric acid (RAM), a chiral compound, is an important intermediate for the chemical synthesis of various antihypertensive and congestive heart failure drugs. Microorganisms capable of converting (R,S)-β-acetylmercaptoisobutyric acid ((R,S)-ester) to RAM were screened from soil microorganisms. A strain of Pseudomonas sp. 1001 screened from a soil sample was selected to be the best. Cells showed an activity of 540 U/mL from culture broth and the enzyme was thermostable up to 70°C. This strain could produce RAM asymmetrically from (R,S)-ester.

ISSN 

1226-8372

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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