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Title 

Suppression of IL-2 and IL-4 gene expression by nodularin through the reduced NF-AT binding activity

Authors 

Sung Su YeaHwan Mook KimJeon Young JinHee Moak OhHye Gwang JeongKyu-Hwan Yang

Publisher 

Elsevier

Issue Date 

2000

Citation 

Toxicology Letters, vol. 114, no. 1, pp. 215-224

Keywords 

T lymphocytesInterleukin-2Interleukin-4NF-AT

Abstract 

Nodularin is a cyclic peptide produced by cyanobacteria. In the present study, the inhibitory effect of nodularin on T lymphocyte functions was demonstrated. Direct addition of nodularin to B6C3F1 mouse splenocyte cultures produced a concentration-dependent inhibition of the lymphoproliferative response to concanavalin A stimulation. Nodularin inhibited PMA plus ionomycin (Io)-induced IL-2 mRNA expression in murine splenocytes and thymocytes as determined by quantitative/competitive RT-PCR. To further characterize the mechanism for the transcriptional regulation of IL-2, the binding activity of transcription factors, NF-AT, AP-1, NF-κB, and Oct, was evaluated by electrophoretic mobility shift assays in mouse splenocytes. Nodularin reduced the NF-AT binding activity in PMA/Io-induced splenocytes, but no significant effect was observed on AP-1, NF-κB, or Oct binding activity. Nodularin also inhibited IL-4 mRNA expression in PMA/Io-stimulated murine splenocytes. These results suggest that T lymphocyte is a possible cellular target of nodularin, and the inhibitory effect of nodularin on T-cell specific transcription factor NF-AT induces T-cell dysfunction, which leads to a diminution in IL-2 and IL-4 gene transcription.

ISSN 

0378-4274

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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