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Title 

Formation of α-D-glucose-1-phosphate by thermophilic α-1,4-D-glucan phosphorylase

Authors 

Hyun Jae ShinY S ShinDae Sil Lee

Publisher 

Springer

Issue Date 

2000

Citation 

Journal of Industrial Microbiology (& Biotechnology), vol. 24, no. 0, pp. 89-93

Keywords 

α-D-glucose-1-phosphateglucose-6-phosphateα-1,4-D-glucan phosphorylasephosphoglucomutasemaltooligosaccharidesThermus caldophilus GK24

Abstract 

For the production of α-D-glucose-1-phosphate (G-1-P), α-1,4-D-glucan phosphorylase from Thermus caldophilus GK24 was partially purified to a specific activity of 13 U mg-1 and an enzyme recovery of 15%. The amount of G-1-P reached maximum (18%) when soluble starch was used as substrate, and the smallest substrate for G-1-P formation was maltotriose. The structure of purified G-1-P was confirmed by comparison to 13C-NMR data for an authentic sample. In addition to G-1-P, glucose-6-phosphate (12%) was simultaneously produced when 10 mM maltoheptaose was used as substrate.

ISSN 

0169-4146

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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