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Title 

A simplified procedure for the purification of human N-acetylglucosaminyltransferase-III from human hepatocellular carcinoma tissues

Authors 

Eun Young SongKyoung A KimYung Dai KimDur Han KwonHong Soo LeeCheorl Ho KimTae Wha ChungSi Myung Byun

Publisher 

Springer Verlag (Germany)

Issue Date 

2000

Citation 

Biotechnology Letters, vol. 22, no. 3, pp. 201-204

Keywords 

hepatocellular carcinomaimmunoaffinity columnmonoclonal antibodyN-acetylglucosaminyltransferase

Abstract 

Human N-acetylglucosaminyltransferase-III (GnT-III) has not been hitherto purified from either tissue or cell line. Although rat GnT-III has been purified from rat kidney tissue, the procedure involves laborious and complex steps. We have developed a simplified procedure based on a QAE (diethyl[2-hydroxypropyl]aminoethyl)-Sepharose and an immunoaffinity chromatography technique. The immunoaffinity chromatography utilized a monoclonal antibody to human aglycosyl recombinant N- acetylglucosaminyltransferase-III. With the new procedure, human N- acetylglucosaminyltransferase-III was purified from hepatocellular carcinoma tissues with an increase in the specific enzyme activity of 378-fold.

ISSN 

0141-5492

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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