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Title 

Brevibacterium ketoglutamicum을 이용한 L-ornithine 생산 연구 part II : L-arginine 제한공급에 의한 L-ornithine 유가식 발효생산

Authors 

Wuk Sang RyuHyung Wook JangHong Weon LeeJoon Ki JungSoon Jae ChangYeon Woo RyuYoung Hoon Park

Publisher 

Korean Journal of Applied Microbiology and Biotechnology

Issue Date 

1999

Citation 

Korean Journal of Applied Microbiology & Biotechnology, vol. 27, no. 4, pp. 327-332

Keywords 

L-ornithineL-argininefed-batch culturecomplex medium

Abstract 

A highly productive fed-batch fermentation process was developed for the production of L-ornithine by using a new stabilized strain, Brevibacterium ketoglutamicum BK52. Fed-batch cultures with a continuous feeding of the complex medium were conducted on various operating conditions. The optimal concentration of phosphate in the complex medium was 2.1 g/L. The optimal feeding rate of L-arginine was 0.028 g/L/hr. The optimal feeding point of the complex medium was determined to be at 40 OD of the cell mass. The final L- ornithine concentrations within 64 hrs of cultivation in 5 and 50 liter fermenters were 73 g/L and 71 g/L, respectively. The maximum overall L- ornithine productivity was 1.14 g/L/hr which was about 2 times higher than that of the conventional fed-batch culture with intermittent feeding. The overall productivity of the fermentation system is remarkably improved by employing the optimized conditions, and it offers a significant potential for industrial application.

ISSN 

0257-2389

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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