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Title | Brevibacterium ketoglutamicum을 이용한 L-ornithine 생산 연구 part II : L-arginine 제한공급에 의한 L-ornithine 유가식 발효생산 |
Authors | Wuk Sang Ryu; Hyung Wook Jang; Hong Weon Lee; Joon Ki Jung; Soon Jae Chang; Yeon Woo Ryu; Young Hoon Park |
Publisher | Korean Journal of Applied Microbiology and Biotechnology |
Issue Date | 1999 |
Citation | Korean Journal of Applied Microbiology & Biotechnology, vol. 27, no. 4, pp. 327-332 |
Keywords | L-ornithine; L-arginine; fed-batch culture; complex medium |
Abstract | A highly productive fed-batch fermentation process was developed for the production of L-ornithine by using a new stabilized strain, Brevibacterium ketoglutamicum BK52. Fed-batch cultures with a continuous feeding of the complex medium were conducted on various operating conditions. The optimal concentration of phosphate in the complex medium was 2.1 g/L. The optimal feeding rate of L-arginine was 0.028 g/L/hr. The optimal feeding point of the complex medium was determined to be at 40 OD of the cell mass. The final L- ornithine concentrations within 64 hrs of cultivation in 5 and 50 liter fermenters were 73 g/L and 71 g/L, respectively. The maximum overall L- ornithine productivity was 1.14 g/L/hr which was about 2 times higher than that of the conventional fed-batch culture with intermittent feeding. The overall productivity of the fermentation system is remarkably improved by employing the optimized conditions, and it offers a significant potential for industrial application. |
ISSN | 0257-2389 |
Appears in Collections | |
Registered Date |
2017-04-19 |
