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Title 

Local structural elements in the mostly unstructured transcriptional activation domain of human p53

Authors 

Hyun LeeK Hun MokRanjith MuhandiramKyu Hwan ParkJae Eun SukDo Hyung KimJun ChangYoung Chul SungKwan Yong ChoiKyou Hoon Han

Publisher 

American Society for Biochemistry and Molecular Biology

Issue Date 

2000

Citation 

Journal of Biological Chemistry, vol. 275, no. 38, pp. 29426-29432

Keywords 

protein p53humanhumanstrans-activation (genetics)tumor suppressor protein p53

Abstract 

DNA transcription is initiated by a small regulatory region of transactivators known as the transactivation domain. In contrast to the rapid progress made on the functional aspect of this promiscuous domain, its structural feature is still poorly characterized. Here, our multidimensional NMR study reveals that an unbound full-length p53 transactivation domain, although similar to the recently discovered group of loosely folded proteins in that it does not have tertiary structure, is nevertheless populated by an amphipathic helix and two nascent turns. The helix is formed by residues Thr18-Leu26 (Thr-Phe-Ser-Asp-Leu-Trp-Lys-Leu-Leu), whereas the two turns are formed by residues Met40-Met44 and Asp48-Trp53, respectively. It is remarkable that these local secondary structures are selectively formed by functionally critical and positionally conserved hydrophobic residues present in several acidic transactivation domains. This observation suggests that such local structures are general features of acidic transactivation domains and may represent 'specificity determinants' (Ptashne, M., and Gann, A. A. F. (1997), Nature 386, 569-577) that are important for transcriptional activity.

ISSN 

0021-9258

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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