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Title 

Cloning of a new Bacillus thuringiensis cry1I-type crystal protein gene

Authors 

Soo Keun ChoiByung Sik ShinEun Mee KongHyune Mo RhoSeung Hwan Park

Publisher 

Springer Verlag (Germany)

Issue Date 

2000

Citation 

Current Microbiology, vol. 41, no. 1, pp. 65-69

Keywords 

bacterial DNAbacterial proteinamino acid sequenceBacillus subtilisBacillus thuringiensisbioassaycrystalEscherichia coliinsecticide resistancemolecular cloning

Abstract 

A new cry1I-type gene, cry1Id1, was cloned from a B. thuringiensis isolate, and its nucleotide sequence was determined. The deduced amino acid sequence of Cry1Id1 is 89.7%, 87.2%, and 83.4% identical to the Cry1Ia, Cry1Ib, and Cry1Ic proteins, respectively. The upstream sequence of the cry1Id1 structural gene was not functional as promoter in B. subtilis. The Cry1Id1 protein, purified from recombinant E. coli cells, had a toxicity comparable to that of Cry1Ia against Plutella xylostella, but it was significantly less active than Cry1Ia against Bombyx mori. Cry1Id1 was not active against the coleopteran insect, Agelastica coerulea.

ISSN 

0343-8651

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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