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Title 

Overexpression of an alkaline lipase gene from Proteus vulgaris K80 in Escherichia coli BL21/pKLE

Authors 

Hong Weon LeeSun Jun YoonHyung Kwon KimKyong Mun ParkTae Kwang OhJoon Ki Jung

Publisher 

Springer Verlag (Germany)

Issue Date 

2000

Citation 

Biotechnology Letters, vol. 22, no. 19, pp. 1543-1547

Keywords 

E. coli BL21/pKLElipaseoverexpression

Abstract 

Over-expression of Proteus vulgaris K80 lipase gene in Escherichia coli BL21 (DE3)/pKLE was achieved, with the enzyme being produced in an as active soluble form, using the T7 RNA polymerase system in a modified M9 salt or M9ZB medium. D-Lactose (55 mM) was used to induce gene expression and gave twice the lipase activity achieved with 0.4 mM IPTG. The expression of the lipase gene depended on the feeding rate of glucose being optimal at 12 g 1-1 h-1.

ISSN 

0141-5492

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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