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Title 

Effect of short interspersed element sequences on the integration and expression of a reporter gene in the preimplantation-stage mouse embryos

Authors 

Yong Kook KangJung Sun ParkChul Sang LeeYoung Il YeomYong Mahn HanAn Sik ChungKyung Kwang Lee

Publisher 

Wiley-Blackwell

Issue Date 

2000

Citation 

Molecular Reproduction and Development, vol. 56, no. 3, pp. 366-371

Keywords 

transgeneSINEintegrationmicroinjection

Abstract 

Based on the assumption that foreign DNA sequences may have increased chance of integration into the host genome if they are flanked by high copy- numbered genomic sequences such as SINEs (short interspersed elements), we investigated the integration frequency of Lac Z reporter gene flanked by a fused B1/B2 in an in vivo system using pronuclear microinjection technique in the mouse. The SINE-flanked DNA showed a 4-fold increased integration frequency of the reporter gene than the control DNA (63% vs. 16%). Moreover, the level of β-galactosidase expression, estimated from the X-Gal staining intensity in transgenic embryos, was greatly higher in SINE-carrying DNA. These results suggest that the SINE sequences can serve a very useful tool in improving the efficiency of current transgenic animal technology.

ISSN 

1040-452X

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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