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Title 

Production of biologically active human granulocyte colony stimulating factor in the milk of transgenic goat

Authors 

Jung Ho KoChul Sang LeeKui Hyun KimMyung Geol PangJa Shin KooNanzhu FangDeog Bon KooKeon Bong OhWoo Sik YounGuo Dong ZhengJung Sun ParkSun Jung KimYong Mahn HanIn Young ChoiJoon Ho LimSang Tae ShinSeung Won JinKyung Gwang LeeOok Joon Yoo

Publisher 

Springer Verlag (Germany)

Issue Date 

2000

Citation 

Transgenic Research, vol. 9, no. 3, pp. 215-222

Keywords 

biological activitybioreactorG-CSFO-glycosylationtransgenic goat

Abstract 

We have developed a transgenic female goat harboring goat beta-casein promoter/human granulocyte colony stimulating factor (G-CSF) fusion gene by microinjection into fertilized one-cell goat zygotes. Human G-CSF was produced at levels of up to 50 μg/ml in transgenic goat milk. Its biological activity was equivalent to recombinant human G-CSF expressed from Chinese hamster ovary (CHO) cell when assayed using in vitro HL-60 cell proliferation. Human G-CSF from transgenic goat milk increased the total number of white blood cells in C57BL/6N mice with leucopenia induced by cyclophosphamide (CPA). The secreted human G-CSF was glycosylated although the degree of O-glycosylation was lower compared to CHO cell-derived human G-CSF.

ISSN 

0962-8819

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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