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Title 

Differential activation of murine macrophages by angelan and LPS

Authors 

Young Jin JeonSang Bae HanKyung Seop AhnHwan Mook Kim

Publisher 

Elsevier

Issue Date 

2000

Citation 

Immunopharmacology, vol. 49, no. 3, pp. 275-284

Keywords 

angelica gigas NakaiangelanmacrophagesNF-κB/RelCD14CR3

Abstract 

In our previous studies, we showed that angelan, a polysaccharide purified from Angelica gigas Nakai, is a potent LPS-mimetic in murine macrophages [Jeon, Y.J., Han, S.B., Ahn, K.S., Kim, H.M., 1999. Activation of NF-κB/Rel in angelan-stimulated macrophages. Immunopharmacology 43, 1-9]. Angelan stimulates murine macrophage to produce cytokines including iNOS and activate NF-κB/Rel. In the present study, we investigated the role of CD14 and complement receptor type 3 (CR3) in mediating NO production and NF- κB/Rel activation induced by angelan and LPS. Three major differences between angelan and LPS were observed. First, angelan does not require serum proteins for NO response and NF-κB/Rel activation, while the activation by LPS requires serum proteins. Second, blocking of either CD14 or CR3 decreased angelan-induced NO response, while LPS-mediated NO production was inhibited by anti-CD14 mAb only. Third, angelan induced strong NF-κB/Rel and slight AP-1 DNA binding, whereas LPS potently activated both NF-κB/Rel and AP-1. Both angelan and LPS degraded IκB proteins and subsequently induced the mobilization of NF-κB/Rel proteins (p65, c-rel and p50) into nucleus. This suggests that macrophages display a common signaling machinery leading to the NF-κB/Rel activation in response to different stimulants. In conclusion, angelan and LPS use the membrane receptor CD14 and CR3 differentially for signaling NF-κB/Rel activation and NO production.

ISSN 

0162-3109

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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