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Title 

Application of fibrin zymography for determining the optimum culture time for protease activity

Authors 

Nack Shick ChoiSeung Ho Kim

Publisher 

Springer

Issue Date 

1999

Citation 

Biotechnology Techniques, vol. 13, no. 0, pp. 899-901

Keywords 

Bacillusfibrinproteasezymography

Abstract 

The culture time for maximal enzyme production by bacteria was determined by SDS-fibrin zymography. Bacillus subtilis and B. amyloliquefaciens secreted several extracellular proteases during the cellular lysis after 40 h and 30 h, respectively. Using this technique, we determined which protein possessed activity and would be isolated before the process of purification began. This proposed method offers an effective harvesting time in which to obtain the proteins having maximal activity.

ISSN 

0951-208X

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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