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Title 

Production of levan using recombinant levansucrase immobilized on hydroxyapatite

Authors 

Ki Hyo JangKi Bang SongJ S KimChul Ho KimBong Hyun ChungSang Ki Rhee

Publisher 

Springer

Issue Date 

2000

Citation 

Bioprocess Engineering, vol. 23, no. 1, pp. 89-93

Keywords 

hydroxyapatitelevanlevansucraserecombinant enzyme

Abstract 

Levansucrase of Zymomonas mobilis was immobilized onto the surface of hydroxyapatite by ionic binding. Optimum conditions for the immobilization were: pH 6.0, 4 h of immobilization reaction time, and 20 U of enzyme/g of matrix. The enzymatic and biochemical properties of the immobilized enzyme were similar to those of the native enzyme, especially towards the effect of salts and detergents. The immobilized enzyme showed sucrose hydrolysis activity higher as that of the native enzyme, but levan formation activity was 70% of the native enzyme. HPLC analysis of levan produced by immobilized enzyme showed the presence of two different types of levan: high-molecular-weight levan and low-molecular-weight levan. The proportion of low-molecular-weight levan to total levan produced by the immobilized enzyme was much higher than that with the native enzyme, indicating that immobilized levansucrase could be applied to produce low-molecular-weight levan. Immobilized levansucrase retained 65% of the original activity after 6 times of repeated uses and 67% of the initial activity after 40 d when stored at 4°C.

ISSN 

0178-515X

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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