상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

Enantioselective hydrolysis of (R,S)-naproxen methyl ester using two-step acetone-treated Candida rugosa lipase

 

2단계 아세톤 침전법으로부터 얻어진 Candida rugosa lipase를 이용한 (R,S)-Naproxen methyl ester의 광학선택성 수화반응

Authors 

Eun Gyo LeeSoon Ja ChoiBong Hyun Chung

Publisher 

Korean Journal of Applied Microbiology and Biotechnology

Issue Date 

2000

Citation 

Korean Journal of Applied Microbiology & Biotechnology, vol. 28, no. 4, pp. 223-227

Keywords 

Candida rugosa lipase (CRL)(S)-naproxen(R,S)-naproxen methyl estertwo-step acetone treatment

Abstract 

A novel two-step acetone treatment method was developed to enhance the enantioselectivity of Candida rugosa lipase (CRL) toward the hydrolysis of racemic naproxen methyl ester. The acetone-treated CRL was considerably more enantioselective than the crude CRL, yielding an enantiomeric excess of 98-100%. The crude and acetone-treated CRLs were subjected to anion exchange chromatography, and their chromatography profiles were compared. In consequence, both chromatography profiles were found to be almost identical, resulting in two separate lipase peaks (lipase A and B). The lipase B, which is known to be less enantioselective, was treated with acetone using a two-step treatment method. The enantioselectivity of acetone-treated lipase B was dramatically increased, yielding an enantiomeric excess of 99 %.

ISSN 

0257-2389

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


There are no files associated with this item.
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)