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Title 

Mouse monoclonal antibodies to hepatitis B virus preS1 produced after immunization with recombinant preS1 peptide

Authors 

Chun Jeih RyuYoun Kyu KimHyang Suk HurHee Sun KimJai Min OhYoung Jun KangHyo Jeong Hong

Publisher 

Mary Ann Liebert

Issue Date 

2000

Citation 

Hybridoma, vol. 19, no. 2, pp. 185-189

Keywords 

monoclonal antibodyrecombinant proteinantibody productionantigen bindingenzyme linked immunosorbent assayhepatitis B virushybridomaimmunizationhepatitis B surface antigens

Abstract 

We have efficiently generated mouse monoclonal antibodies (MAbs), which bind specifically to amino acids 21-47 of the preS1 domain of hepatitis B virus (HBV) by immunizing mice with the preS1 peptide (amino acids, aa 1-56) conjugated to keyhole limpet hemocyanin. Hybridomas were screened by an indirect enzyme-linked immunosorbent assay (ELISA) using the purified preS1 peptide as a coated antigen. Eighteen positive hybridomas were selected and subjected to isotyping. of these, 5 clones secreted immunoglobulin G (IgG) and 13 clones secreted IgM. Four (KR1, KR2, KR3, and KR4) of the 5 IgG MAbs bound to preS1 peptide (aa 21-47). Epitope mapping using bacterially expressed GST fusion proteins revealed that three clones (KR2, KR3, KR4) (IgG1, κ) recognize aa 21-35, while KR1 (IgG2a, κ) recognizes aa 35-47 of the preS1. These MAbs immunoprecipitated HBV particles, demonstrating that they bind to native HBV particles.

ISSN 

0272-457X

Link 

http://dx.doi.org/10.1089/02724570050031248

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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