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Title 

Role of Lys335 in the metastability and function of inhibitory serpins

Authors 

Ha Na ImMyeong Hee Yu

Publisher 

Cold Spring Harbor Laboratory Press

Issue Date 

2000

Citation 

Protein Science, vol. 9, no. 5, pp. 934-941

Keywords 

α1-antitrypsininhibitory mechanismlysinemetastabilityserine protease inhibitorsstrain

Abstract 

The native form of inhibitory serpins (serine protease inhibitors) is not in the thermodynamically most stable state but in a metastable state, which is critical to inhibitory functions. To understand structural basis and functional roles of the native metastability of inhibitory serpins, we have been characterizing stabilizing mutations of human α1-antitrypsin, a prototype inhibitory serpin. One of the sites that has been shown to be critical in stability and inhibitory activity of α1-antitrypsin is Lys335. In the present study, detailed roles of this lysine were analyzed by assessing the effects of 13 different amino acid substitutions. Results suggest that size and architect of the side chains at the 335 site determine the metastability of α1-antitrypsin. Moreover, factors such as polarity and flexibility of the side chain at this site, in addition to the metastability, seem to be critical for the inhibitory activity. Substitutions of the lysine at equivalent positions in two other inhibitory serpins, human α1- antichymotrypsin and human antithrombin III, also increased stability and decreased inhibitory activity toward α-chymotrypsin and thrombin, respectively. These results and characteristics of lysine side chain, such as flexibility, polarity, and the energetic cost upon burial, suggest that this lysine is one of the structural designs in regulating metastability and function of inhibitory serpins in general.

ISSN 

0961-8368

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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